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Lactose, which is a disaccharide of course found in milk and dairy merchandises, supplies about 40 % energy demands for human babes. However, due to a deficiency of Lactaid enzyme in the digestive system, lactose can non be broken down in the little bowel and so will ferment in the big bowel, bring forthing lactic acid and H gas. This consequences in terrible uncomfortableness, spasms, bloating, air current, and diarrhea.

Lactose intolerance can take to serious complications in an baby, such as relentless diarrhea and failure to derive weight. Babies frequently suffer from transient lactose intolerance, particularly after an infection. Harmonizing to recent surveies in the Department of Paediatrics, Guy ‘s Hospital, London, and a study on the British Journal of Community Nurses have shown that about half the babes suffered colic due to lactose intolerance. In most instances, impermanent lactose intolerance resolves itself within four hebdomads, although some babies take up to eight hebdomads to to the full retrieve.

Following research at Guys Hospital, which successfully identified transeunt Lactaid lack as one possible cause of Colic, Colief® Infant Drops are progressively being prescribed by Doctors on the NHS. Nonetheless, many parents have reported that Colief® does non look to work on their babes. ( sjfksjd )

Research AND RATIONALE:

The probe is to prove whether Colief plants or non. ? ? ?

Briefly, Lactaid interruptions lactose into glucose and brain sugar:

I would mensurate the alterations in lactose concentration after adding the enzyme into milk solutions but in fact I did non hold the equipments to mensurate lactose content. Therefore a blood glucose metre was used alternatively. Concentration of glucose formed due to lactose was broken down can be so determined by the metre.

However, the metre needed to be checked that it was working good.

Planning:

Equipments:

ACCU-CHEK Aviva blood glucose metre from Roche.

Test trips for Accu-chek Aviva

Normal semi-skimmed milk

Solgar Lactase 3500 tablets

Colief® Infant Drops

Thermometer ( to keep the temperature )

Measuring panpipes

Electric Balance

Measuring cylinders

Method

Diluted the milk solution by adding H2O to milk until a point, where a suited glucose concentration is reached- in reading scope of the metre. Every clip the milk was diluted, the glucose concentration was checked.

Put the milk solution in a glass beaker. Then place the beaker in a H2O bath ( in this instance – was a jug )

Keep exceeding up hot H2O to the jug to keep the temperature ( at 370C ) . A quicksilver thermometer was used to look into the temperature in between.

Glucose concentration of milk solutions were tested before and after adding the enzyme. Carefully dipped the top of the trial strip into the solution.

The information was recorded every one minute up to the 5th minute and every five proceedingss up to the 35th or 40th minute.

Plot the information. The alteration in the concentrations would turn out whether milk sugar had been broken down and the enzyme did worked.

Hazard appraisals:

I used the boiling H2O to add to the jug to maintain the temperature the same – I had to be really careful when making that. I did take everything unneeded that can by chance be broken.

Glass beakers were use alternatively of the plastic 1s – because the fictile 1s ‘ are heat insulate – do the milk solution ‘s temperature different. I besides had to be really careful when making this. Otherwise I might hold broken the beaker.

It besides needed to be highly careful when working with the thermometer as when it broken the quicksilver can leak out which is really unsafe and toxic.

Practical:

A test experiment was carried out to do certain the metre worked and to happen out the suited step scope of the metre.

Using semi-skimmed milk.

Dilution of milk

Meter ‘s Reading

Neat

Excessively high

1 in 2 ( 5 milliliter milk + 5 ml H2O )

Excessively high

1 in 4 ( 5 milliliter milk + 15 ml H2O )

Excessively high

1 in 8 ( 5 milliliter milk + 35 ml H2O )

26.0 mmol/l

1 in 16 ( 5 milliliter milk + 75 ml H2O )

13.5 mmol/l

1 in 32 ( 5 milliliter milk + 155 ml H2O )

07.2 mmol/l

A farther measure was needed to look into whether the metre was mensurating specifically the glucose itself or included glucose in the disaccharides, excessively.

Sucrose, which is besides a disaccharide, is made of glucose and galactose. A solution of saccharose was tested with the metre. A negative consequence of 0 mmol/l proved that the metre merely measured glucose in milk.

( Protein – 0.1ml = 20.6mg )

7 milliliter = 160 beads. 4 beads each clip = 0.175ml – milk has been diluted – 0.1ml is used alternatively.

The 2nd test was to gauge how long it would take for the enzyme to interrupt down all the milk sugars:

A land Soglar tablet was added into milk solution.

Dilution of milk

Reading

Neat

30.3 mmol/l

1 in 2 ( half cup of milk + half cup of H2O )

17.9 mmol/l

After adding Soglar Lactaid 10 mins

26.5 mmol/l

After adding Lactaid 40 mins

Excessively high

From the two tests, we knew that milk solution needed to be diluted and the measurement clip for each solution was about 40 proceedingss.

Consequences:

With Lactose-free milk: as

The readings following were recorded at 200C

Milk dilution

Glucose concentration ( mmol/L )

5ml milk + 35ml H2O

32.2

5ml milk + 75 milliliter H2O

17.5

As the consequence, dilution of 1 in 16 was used.

The setup was set up precisely the same as the old one.

Temperature was kept at 370C.

0.1 ml Colief added.

Glucose Concentration

( mmol/l )

17.8

18.1

18.6

18.3

Time ( proceedingss )

0

5

10

15

I decided to halt at the fifteenth minute as the glucose concentration seemed non to alter any longer.

There should hold been no alteration in glucose concentration in this milk solution because it is lactose-free, which means no milk sugar would hold been broken down by the enzyme – no glucose made. However, the consequence showed there was a little alteration in glucose concentration. This may be due to systematic mistake of the metre.

Semi-skimmed milk

First, the milk was diluted.

Dilution of semi-skimmed milk

Glucose concentration

5ml milk + 5ml H2O

26.6 mmol/l

5ml milk + 15 milliliter H2O

18.1 mmol/l

5ml milk + 35 milliliter H2O

8.9 mmol/l

As the consequence, the dilution 1 in 8 was applied to another two processs.

0.1ml of Colief was added.

Time/

Minutess

Glucose concentration ( mmol/L )

No. 1

No. 2

No. 3

Average

0

8.9

7.8

6.8

7.8

1

12.7

11.3

10.6

11.5

2

15.0

13.2

14.2

14.1

3

17.5

15.7

16.7

16.6

4

19.6

17.5

19.3

18.8

5

23.1

19.1

21.4

21.2

10

26.3

22.8

23.6

24.2

15

28.8

25.5

27.4

27.2

20

29.1

25.2

30.4

28.2

25

30.3

26.1

31.4

29.3

30

29.2

26.6

27.9

35

30.2

25.4

27.8

1 Soglar tablet was added:

Glucose Concentration

( mmol/l )

6.9

14.7

20.6

22.6

25

27.7

28.6

30.6

30.9

Time ( proceedingss )

0

5

10

15

20

25

30

35

40

Remark: Unite the consequences of two experiments ;

When I ground and added the tablet to the milk solutions, the tablet merely partly dissolved. In add-on, the sum of enzyme calculated in one Soglar tablet is much more than in 0.1ml of Colief.

we can see that the tablet is non suggested to give to babes as it is non every bit effectual as the Colief liquid.

With SMA babe milk:

The readings following were recorded at 200C

Milk dilution

Glucose concentration ( mmol/L )

5ml milk

Excessively high

5ml milk + 35 milliliter H2O

12.3

As the consequence, dilution of 1 in 8 was used.

The setup was set up precisely the same as the old one.

Temperature was kept at 370C.

0.1 ml Colief added.

Time/

Minutess

Glucose concentration ( mmol/L )

No. 1

No. 2

No. 3

Average

0

14.8

14.4

15.4

14.9

1

15.0

15.9

13.6

15.3

2

16.4

14.8

14.8

15.7

3

17.6

16.2

14.8

15.6

4

15.9

17.0

14.3

16.2

5

17.4

17.0

14.3

16.6

10

18.4

17.3

15.4

17.2

15

18.8

18.9

16.0

17.4

20

17.4

16.8

17.0

17.2

25

17.8

16.5

18.9

18.0

30

18.5

17.3

17.0

17.4

35

17.9

18.3

17.8

18.3

40

18.9

17.9

17.5

17.7

The consequences of these experiments with SMA expression babe milk were unexpected. The glucose concentration kept fluctuating. This led to inquiries: Was there any job with the metre, was at that place anything that interfered the metre? Or was the enzyme inhibited?

… … … … … … … … … .

In the experiments, the temperature was kept at 37oC – this is disposed for human temperature and meets the needed temperature for the Colief to work decently.

About the pH, milk shows innate ability to defy the alterations in the pH – its buffering capacity ( this is chiefly due to the presence of amino acid residues of caseins and whey protein and colloidal salts ) . Therefore I did non worry to keep the pH as the milk could make it itself.

The consequences can turn out that Colief is non truly effectual – and this could be the ground why high proportions of parents holding flatulent babes ( cause by transient lactose intolerance ) have reported Colief did non work for their babes.

During the research I came across some merchandises that were besides recommended for flatulent babes as Infacol, Lactase Enzyme Liquid.

I will make other experiments with low pH – like in our tummy environment – so I can cognize whether the enzyme will work in grownup ‘s tummy when tablet ( like Soglar ) is swallowed

Post Author: admin