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In the present survey, three batches of different marketed polyherbal preparation. Trifla Churna were produced from the local market and they were evaluated as per Indian Pharmacopoeia and WHO guidelines on the undermentioned parametric quantities viz, Organoleptic features, Extractive value, Ash value, Physical characteriristics, Moisture content, Loss on drying, Phytochemical rating, Fluorescence analysis, pH value etc. The consequence of Trifla Churna was found in close propinquity. This survey on Trifla Churna was precise, consistent and may be considered as a protocol for its rating.

Cardinal WORDS: Trifla Churna, Polyherbal preparation, Protocol and

Quality Control

Introduction

The recent involvement in Ayurvedic system of medical specialty is seen by big scale fabrication of ayurvedic preparation must conform to the trial for individuality, authority, pureness, safety and efficaciousness. Majority of the ayurvedic preparations use whole workss either entirely or in combination. It has been stated that uniting herbs improves efficaciousness and reduces inauspicious effects due to the low concentration of active ingredients adequate to bring forth therapeutics effects but to cut down the toxicity. Inspite of the big figure of ayurvedic preparation available in market, for many of them, criterion for their quality are yet to be laid. Assorted marketed preparation shows dose fluctuation, content fluctuation and deficiency of standardisation which affects its curative activity, therefore it is imperative to develop fast, sensitive and accurate methods of analysis for ayurvedic preparations which will be in alignment with modern engineering.

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This paper includes the probe of Quality Control methods for three different samples of Trifla Churna designated as TC1, TC2, and TC3. The preparation is official in the ayurvedic literatures and therapeutically utile in the intervention of chronic febrilities, peptic ulcers, perspiration, malaria, loss of appetite etc. It is one of celebrated ayurvedic preparation containg more than 40 different ingredients. Different fabrication companies used different active ingredient for their readying, which are non claimed on the container due to which their efficaciousness can non be entree accurately. Therefore, the present survey was undertaken to measure Trifla Churna as per Indian Pharmacopoeia and WHO guidelines.

EXPERIMENTAL

Three marketed preparations of Trifla Churna from different industries ( designated as TC1, TC2 and TC3 ) were produced for the present survey. Alcohol, Acetone, Methanol, n-Hexane, Chloroform, 1N hydrochloric acid, 1N Na chloride ( aqueous ) , ferrous chloride, 1N azotic acid, ammonium hydroxide, I, 1N Na hydrated oxide ( alcoholic ) , picric acid and 1N sulfuric acid etc.

Organoleptic belongingss of Trifla Churna: Organoleptic belongingss of each of three batches of Triphla Churna were done by utilizing reported methods.

Extractive values: 5 g of Trifla Churna from each batch for single extraction of extracted with n-hexane, trichloromethane, methyl alcohol, and distilled H2O individually by cold maceration method described below and their extractive values were determined as per the methods given in Indian Pharmacopoeia and WHO publication.

n-Hexane and Chloroform soluble extractives: n-Hexane and Chloroform soluble extractives were determined by same process as described above and dried under decreased force per unit area.

Methanol soluble extractives: Trifla Churna was dispersed in 100 milliliter of Methanol and allows it to stand for 24 hour with occasional shaking. Extract was filtered and evaporated.

Water soluble extractives: Trifla Churna was dispersed in 100 milliliter of Water and allows it to stand for 24 hour with occasional shaking and filtered. The above process was performed for each batch and the dried H2O extractives of TC1, TC2 and TC3 were weighed.

The extractive values of the all three batches of Trifla Churna in above dissolver are given in Table-1.

Ash values: Entire ash, acid indissoluble ash and H2O soluble ash values were determined utilizing standard process. ( Table-2 )

Physical features: The physical features of the Trifla Churna were determined for TC1, TC2 and TC3 in footings of the majority denseness, true denseness, angle of rest, hausner ‘s ratio and Carr ‘s index harmonizing to the standard process. ( Table-3 )

Moisture content and Loss on drying: Moisture content and Loss on drying was determined for all three batches of Triphla Churna as per standard process. ( Table-4 )

Phytochemical rating: For this survey, aqueous infusion of Trifla Churna has been employed, testing procedure of each batch of Trifla Churna for phytochemical rating was done utilizing reported methods. ( TYable-5 )

Fluorescence analysis: For fluorescence analysis, the drug pulverization was treated with different dissolver in different trial tubing. The dissolvers used were 1N HCL, 1N NaOH ( aqueous ) , FeCl3, 1N HNO3, NH3, I2, 1N NaOH ( alcoholic ) , picric acid and 1N H2SO4. Then they were subjected to fluorescence analysis in daytime and in UV visible radiation as per standard process. ( Table-6 )

pH finding: The pH values of 1 and 10 % ( w/v ) solution of different batches of Trifla Churna were determined as per I.P. ( Table-7 )

Foreign affair, frothing index and swelling index: Foreign affair, frothing index and swelling index were determined for all three batches of Trifla Churna as per standard process.

RESULTS AND DISCUSSION

Trifla Churna ( three batches, TC1, TC2 and TC3 ) was evaluated in the research lab harmonizing to standard processs. They were evaluated by comparative analysis for their organoleptic belongingss, Extractive values ( n-hexane, trichloromethane, methyl alcohol, and H2O ) , Ash values ( Entire ash, acid indissoluble ash and H2O soluble ash ) , Physical features, Moisture content, Loss on drying, Phytochemical rating, Fluorescence analysis, pH value, Foreign affair, frothing index and swelling index.

Organoleptic surveies revealed that all the three batches ( TC1, TC2 and TC3 ) of Trifla Churna were brown in coloring material, holding pleasant smell and possessing acrid gustatory sensation. More than 90 % of two samples ( TC1 and TC2 ) passed through 60-mesh screen except MSC3 passed60 % merely. It was besides observed that more than 40 % of all the samples passed through 85-mesh screen.

Extractive values are reported in table-1 and ash values are included in table-2. The extractive values ( % w/w ) of Trifla Churna ( Mean ± SD of TC1, TC2 and TC3 ) in n-hexane and trichloromethane were found to be 4.39 ± 0.63 and 14.08 ± 1.70, severally, indicative the presence of polar and semi polar constitutes in Trifla Churna. The ash value of each batch of Trifla Churna for entire ash, acid indissoluble ash and H2O soluble ash are found to be 70.38 ± 2.93, 45.08 ± 3.73 and 51.31 ± 2.39, severally, which indicates the presence of inorganic affairs as major constituents.

TABLE-I: EXTRACTIVE VALUES OF TRIFLA CHURNA

Extractive

Values* % w/v ( Mean ± SD )

TC1

TC2

TC3

Water soluble

Methanol soluble

n-Hexane

Chloroform

*Values of mean of three experiments.

TABLE-II: ASH VALUES OF TRIFLA CHURNA

Samples

Ash values* % ( Mean ± SD )

TC1

TC2

TC3

Entire ash

Water soluble ash

Acid indissoluble ash

*Values of mean of three experiments.

The physical features of all the samples of Trifla Churna were shown in Table-3. Low values of angle of repose show the hapless flow ability for all samples. The consequences of the three batches were found to be comparable. The % wet content and loss on drying values of Trifla Churna are reported in Table-4. TABLE-III: Physical CHARACTERISTICS OF DIFFERENT FORMULATION OF TRIFLA CHURNA

Parameters

Values* % ( Mean ± SD )

TC1

TC2

TC3

Bulk denseness ( gmL )

True denseness ( gmL )

Angle of rest ( – )

Hausner ‘s ratio

Carr ‘s index

*Values of mean of three experiments.

These probes of Phytochemical rating are shown in Table-5 which shows the presence of different phytoconstituent. The fluorescence analysis ( Table-6 ) revealed that each of the three batches of Trifla Churna are non showed the major distinguishable fluorescing belongings.

Table -IV: MOISTURE CONTENT AND FOREIGN MATTER OF TRIFLA CHURNA

Samples

Values* % ( Mean ± SD )

Moisture content

Loss on drying

TC1

TC2

TC3

*Values of mean of three experiments.

TABLE-V: PHYTOCHEMICAL EVALUATION OF TRIFLA CHURNA

Phytoconstituents

TC1

TC2

TC3

Alkaloids

Glycosides

Phytosterols

Essential oil

Tannins

Saponins

Proteins

TABLE-VI: POWDER FLUORESCENCE ANALYSIS OF TRIFLA CHURNA

Material

TC1

TC2

TC3

Day

Light

UV 254nm

Day

Light

UV 254nm

Day

Light

UV 254nm

Powder as such

P + 1N HCL

P + 1N NaOH ( a )

P + FeCL3

P + 1N HNO3

P + Ammonia

P + Iodine

P + Picric acid

P + 1N H2S04

BR=Brown, YBR=Yellowish brown DBR=Dark brown LBR=Light brown BBR=Blackish brown B=Black Y=Yellow FY=Florescent yellow

The pH values of 1 and 10 % w/v solutions of all the batches were included in Table-7, which showed that Trifla Churna is acidic in nature. No foreign affair was found and the consequence of foaming and swelling index reveals the absence of saponin and mucilage in all three batches of Trifla Churna. The information analysis reveals that all parametric quantities remain in close propinquity for each batch of Trifla Churna.

TABLE-VII: pH OF 1 AND 10 % w/v SOLUTION OF DIFERENT FORMULATION OF TRIFLA CHURNA

Different preparations

1 % ( w/v ) concentration ( Mean ± SD ) , n=3

10 % ( w/v ) concentration ( Mean ± SD ) , n=3

TC1

TC2

TC3

No attending has been paid to measure a polyherbal preparation by comparative analysis. The deliberate parametric quantities and the developed methods for their finding can be considered as a protocol for the rating of Trifla Churna, which will help the regularetory governments, scientific organisation and makers in developing criterions. The method used for rating is found to be precise and consistent and aid to bring forth unvarying criterion merchandises, which will reconstruct religion in Ayurvedic system.

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